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Expression of NG2 proteoglycan during endochondral and intramembranous ossification
Author(s) -
Fukushi Junichi,
Inatani Masaru,
Yamaguchi Yu,
Stallcup William B.
Publication year - 2003
Publication title -
developmental dynamics
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.634
H-Index - 141
eISSN - 1097-0177
pISSN - 1058-8388
DOI - 10.1002/dvdy.10359
Subject(s) - intramembranous ossification , endochondral ossification , biology , cartilage , proteoglycan , microbiology and biotechnology , ossification , alkaline phosphatase , anatomy , endocrinology , medicine , biochemistry , enzyme
We have used immunohistochemistry to study the distribution of the NG2 proteoglycan during bone development in the mouse. At embryonic day 15.5, NG2 was strongly detected in the immature cartilage of developing limbs. After transient down‐regulation in mature chondrocytes, NG2 was up‐regulated during primary ossification, colocalizing with alkaline phosphatase and tenascin C. In the epiphyseal growth plates of newborn mouse tibia, NG2 and alkaline phosphatase exhibited overlapping patterns of expression by hypertrophic chondrocytes and by osteoblasts surrounding newly formed bone trabeculae. NG2 was down‐regulated after puberty, being only faintly detectable in the tibial growth plates of 3‐month‐old mice. In cranial sutures, NG2 was strongly labeled in osteogenic bone fronts and in the suture matrix. Our results indicate that NG2 expression is up‐regulated during both endochondral and intramembranous ossification, but is down‐regulated as ossification is completed. Developmental Dynamics 228:143–148, 2003. © 2003 Wiley‐Liss, Inc.

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