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Pluripotency of reprogrammed somatic genomes in embryonic stem hybrid cells
Author(s) -
Tada Masako,
Morizane Asuka,
Kimura Hironobu,
Kawasaki Hiroshi,
Ainscough Justin F.X.,
Sasai Yoshiki,
Nakatsuji Norio,
Tada Takashi
Publication year - 2003
Publication title -
developmental dynamics
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.634
H-Index - 141
eISSN - 1097-0177
pISSN - 1058-8388
DOI - 10.1002/dvdy.10337
Subject(s) - biology , somatic cell , embryonic stem cell , reprogramming , stem cell , cellular differentiation , microbiology and biotechnology , genome , genetics , cell , gene
Somatic nuclei can be epigenetically reprogrammed by factors present in undifferentiated embryonic stem (ES) cells. The acquisition of pluripotency by somatic genomes could render such cells a viable source of personalized cell type(s) for therapeutic application, avoiding the need for controversial therapeutic cloning. To investigate this possibility, we first determined the origin of transcripts in teratomas generated from mouse (ES × somatic cell) hybrid clones. Transcription of markers from the somatic genome demonstrated efficient in vivo differentiation down independent lineages. The induction of dopaminergic neurons by coculture with stromal PA6 feeder cells also demonstrated efficient capacity to differentiate in vitro. Hybrid clone‐derived neurons expressed appropriate markers, and transcription of Pitx3 from the somatic genome was confirmed. When transplanted into mouse brains, the dopaminergic neurons were successfully integrated and expressed tyrosine hydroxylase. Thus, it should be possible to produce personalized ES‐like cells with the reprogrammed somatic genomes. Developmental Dynamics 227:504–510, 2003. © 2003 Wiley‐Liss, Inc.