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Hoxb1 neural crest preferentially form glia of the PNS
Author(s) -
Arenkiel Benjamin R.,
Gaufo Gary O.,
Capecchi Mario R.
Publication year - 2003
Publication title -
developmental dynamics
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.634
H-Index - 141
eISSN - 1097-0177
pISSN - 1058-8388
DOI - 10.1002/dvdy.10323
Subject(s) - rhombomere , neural crest , biology , hox gene , cranial neural crest , population , neural fold , fate mapping , microbiology and biotechnology , anatomy , neuroscience , genetics , neural plate , embryo , gene , gene expression , embryonic stem cell , demography , sociology
The vertebrate cranial neural crest cells give rise to many complex derivatives of the head, neck, and face, including neuronal and glial cells that act in concert for proper development of the anterior–peripheral nervous system. Several genes have been implicated in the processes of neural crest specification, migration, and differentiation; among these are the hox gene clusters. To determine the fates of hox‐expressing cranial neural crest, we describe the results of a genetic lineage analysis by using the Cre/loxP system to drive the activation of different ROSA26 reporter alleles under the regulation of the hoxb1 locus. By targeting the 3′ untranslated region of the hoxb1 gene, we have preserved endogenous gene activity and have been able to accurately follow the fates of the cells derived from the hoxb1 expression domain. Emphasis was placed on identifying the cell and tissue types that arise from the rhombomere 4‐derived neural crest. Our results demonstrate that, in addition to forming much of the cartilage, bones, and muscle of the ears and neck, a significant population of rhombomere 4‐derived neural crest is fated to generate the glial component of the seventh cranial nerve. Developmental Dynamics 227:379–386, 2003. © 2003 Wiley‐Liss, Inc.