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Differential expression of β3 integrin gene in chick and mouse cranial neural crest cells
Author(s) -
Pietri Thomas,
Thiery Jean Paul,
Dufour Sylvie
Publication year - 2003
Publication title -
developmental dynamics
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.634
H-Index - 141
eISSN - 1097-0177
pISSN - 1058-8388
DOI - 10.1002/dvdy.10299
Subject(s) - biology , neural crest , cranial neural crest , microbiology and biotechnology , neural fold , gene , anatomy , gene expression , genetics , neural plate , embryo
RNA in situ hybridization on early chicken embryos revealed that the β3 integrin gene started to be expressed after Hamburger and Hamilton (HH) stage 6 in the presumptive epidermis adjacent to the neural plate, before closure of the neural tube. The β3 integrin gene was also strongly expressed in cephalic neural crest cells at the same stage in which they begin their migration but disappeared progressively in these cells along the route they take to the branchial arches. The gene was weakly expressed in the differentiating cranial neural crest cells. The αVβ3 integrin protein complex was also mainly detected in the migratory cephalic neural crest cells. However, during early mouse embryogenesis and in contrast to the chick, the β3 integrin gene was expressed in the foregut diverticulum and in the heart and not in the cephalic neural crest cells. Therefore, the difference in the β3 integrin expression suggests that mouse and chicken cranial neural crest cells may have distinct integrin requirements during their ontogenesis. Developmental Dynamics 227:309–313, 2003. © 2003 Wiley‐Liss, Inc.