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Isolation and characterization of Xenopus Hey‐1 : A downstream mediator of Notch signaling
Author(s) -
Rones M.S.,
Woda J.,
Mercola M.,
McLaughlin K.A.
Publication year - 2002
Publication title -
developmental dynamics
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.634
H-Index - 141
eISSN - 1097-0177
pISSN - 1058-8388
DOI - 10.1002/dvdy.10192
Subject(s) - biology , xenopus , mediator , notch signaling pathway , microbiology and biotechnology , isolation (microbiology) , downstream (manufacturing) , anatomy , signal transduction , bioinformatics , genetics , gene , engineering , operations management
Regulation of Notch signaling likely occurs, at least in part, at the level of basic helix‐loop‐helix (bHLH) transcription factors that function downstream of Suppressor of Hairless (Su(H)) in the Notch pathway. To begin to characterize modulation of Notch signaling during organogenesis, we examined the bHLH transcription factor, XHey‐1 ( hairy related‐1 ) in early Xenopus laevis embryos. XHey‐1 is expressed in numerous tissues during early development including the somites, head, embryonic kidneys, and heart. Importantly, the expression of XHey‐1 was significantly altered in response to perturbation of Notch signaling by means of inducible constructs that served to either activate or suppress Notch signaling through Su(H) in a temporally controlled manner. © 2002 Wiley‐Liss, Inc.