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Prep2 : Cloning and expression of a new prep family member
Author(s) -
Haller Klaus,
Rambaldi Isabel,
Kovács Erzsébet Nagy,
Daniels Eugene,
Featherstone Mark
Publication year - 2002
Publication title -
developmental dynamics
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.634
H-Index - 141
eISSN - 1097-0177
pISSN - 1058-8388
DOI - 10.1002/dvdy.10167
Subject(s) - biology , homeobox , in situ hybridization , cloning (programming) , microbiology and biotechnology , genetics , gene , organogenesis , gene expression , computer science , programming language
Abstract We describe Prep2 , a new murine homeobox‐containing gene closely related to Prep1 . The PREP2 protein belongs to the three amino acid loop extension (TALE) superclass of homeodomain‐containing proteins and encodes a polypeptide of 462 residues. As for PREP1, PREP2 binds an appropriate site on DNA as a heterodimer with PBX1A. Northern analysis, immunoblotting, immunohistochemistry, and in situ hybridization show widespread Prep2 expression during organogenesis and in the adult. The data suggest that Prep2 functions to varying degrees in a broad array of tissues and developmental processes. © 2002 Wiley‐Liss, Inc.