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RP59, a marker for osteoblast recruitment, is also detected in primitive mesenchymal cells, erythroid cells, and megakaryocytes
Author(s) -
Krüger Anders,
Ellerström Catharina,
Lundmark Carin,
Christersson Cecilia,
Wurtz Tilmann
Publication year - 2002
Publication title -
developmental dynamics
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.634
H-Index - 141
eISSN - 1097-0177
pISSN - 1058-8388
DOI - 10.1002/dvdy.10067
Subject(s) - biology , mesoderm , bone marrow , primitive streak , haematopoiesis , mesenchymal stem cell , yolk sac , cd34 , ectoderm , microbiology and biotechnology , pathology , stem cell , immunology , embryonic stem cell , embryo , embryogenesis , medicine , biochemistry , gene
We recently described a novel protein in bone marrow of rats, RP59, as a marker for cells with the capacity to differentiate into osteoblasts. In this work, its expression pattern was further investigated to learn about the origin and biological relevance of RP59 expressing marrow cells. As revealed by in situ hybridization and by immunohistochemistry of yolk sac embryos, RP59 was found in the cells of the primitive ectoderm and primitive streak as well as in blood islands and extraembryonal mesoderm. Later, RP59 occurred in fetal liver cells and in circulating blood. From the time around birth, it was found in bone marrow and spleen cells. In addition, in vitro–formed blood vessels contained RP59‐positive cells in the lumen. Endothelial cells and the vast majority of cells outside the blood vessels were not labeled. Concerning more mature hematopoietic cell types, RP59 was observed in megakaryocytes and nucleated erythroblasts, but absent from lymphoid cells. In conclusion, RP59 was induced in early mesoderm. It was maintained in the erythroid and megakaryotic lineages and, as earlier described, in young osteoblasts. © 2002 Wiley‐Liss, Inc.