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The analysis of the transcriptome as a new approach for biomarker development to trace the abuse of anabolic steroid hormones
Author(s) -
Riedmaier Irmgard,
Pfaffl Michael W.,
Meyer Heinrich H. D.
Publication year - 2011
Publication title -
drug testing and analysis
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.065
H-Index - 54
eISSN - 1942-7611
pISSN - 1942-7603
DOI - 10.1002/dta.304
Subject(s) - anabolism , anabolic steroid , transcriptome , hormone , anabolic agents , steroid , computational biology , biomarker , biology , bioinformatics , gene expression , endocrinology , gene , biochemistry
The abuse of anabolic steroid hormones in human sports and animal husbandry is an ubiquitous problem and therefore a tight control program in both areas is very important. Within these control programs, hormone residues are detected by immunoassays or chromatographical methods in combination with mass spectrometry. With these methods, all known substances can be detected; yet new xenobiotic growth promoters and new ways of application are difficult to detect. Therefore it is important to develop new sensitive screening methods to enable an efficient control for misused anabolic substances. The detection of their physiological action is a promising approach. Anabolic steroid hormones directly influence the expression of specific genes and thus the analysis of the transcriptome of different target tissues and matrices is of great interest. This review describes our recent efforts made concerning the analysis of gene expression changes in different tissues, different species and under different anabolic treatments. Copyright © 2011 John Wiley & Sons, Ltd.