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Towards an untargeted mass spectrometric approach for improved screening in equine antidoping
Author(s) -
Keen Bethany,
Cawley Adam,
Fouracre Chris,
Pyke James,
Fu Shanlin
Publication year - 2021
Publication title -
drug testing and analysis
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.065
H-Index - 54
eISSN - 1942-7611
pISSN - 1942-7603
DOI - 10.1002/dta.3021
Subject(s) - workflow , chromatography , butorphanol , food and drug administration , substance abuse detection , mass spectrometry , chemistry , pharmacology , drug , medicine , computer science , database
The emergence of novel doping agents is a continuous issue for analysts who aim to maintain the integrity of horseracing together with the well‐being and safety of the animals and riders involved. Untargeted mass spectrometric analysis presents a potential improvement for antidoping as it enables the detection of compounds being indirectly affected by an administered drug. In this study, liquid chromatography–high‐resolution mass spectrometry was used to investigate a 12‐horse administration study of the synthetic opioid, butorphanol. A mass spectrometric workflow capable of detecting metabolic differences for an extended period of time was successfully developed. This proof‐of‐concept study demonstrates the potential of untargeted workflows to provide a list of biomarkers of exposure and effect that are indicative of drug administration which may be implemented into routine testing for improved doping control.