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A sensitive flow injection chemiluminescence method for the determination of progesterone
Author(s) -
Cao Wei,
Gong Pixue,
Liu Wenwen,
Zhuang Mei,
Yang Jinghe
Publication year - 2013
Publication title -
drug testing and analysis
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.065
H-Index - 54
eISSN - 1942-7611
pISSN - 1942-7603
DOI - 10.1002/dta.299
Subject(s) - chemiluminescence , potassium permanganate , detection limit , chemistry , relative standard deviation , chromatography , ruthenium , permanganate , analytical chemistry (journal) , inorganic chemistry , catalysis , biochemistry
A sensitive flow injection (FI) chemiluminescence (CL) method was developed for the determination of trace amounts of progesterone. This method was based on the luminescent properties of the tris(1,10‐phenanthroline) ruthenium(II) ‐ potassium permanganate (KMnO 4 ) ‐ progesterone in acidic medium sensitized by Na 2 SO 3 . With the peak height as a quantitative parameter applying optimum conditions, the relative CL intensity was linear with progesterone concentration in the range of 1.0 × 10 −10 ∼ 6.0 × 10 −9 g·ml −1 and 6.0 × 10 −9 ∼ 4.0 × 10 −8 g·ml −1 with a detection limit of 7.1 × 10 −11 g·ml −1 . The relative standard deviation (RSD) was 2.79% for 1.0 × 10 −8 g·ml −1 progesterone ( n = 11). The proposed method held low detection limit and was successfully applied to determination of progesterone in pharmaceutical preparations. The possible CL reaction mechanism was also discussed. Copyright © 2011 John Wiley & Sons, Ltd.