Premium
The intra‐individual stability of GH biomarkers IGF‐I and P‐III‐NP in relation to GHRH administration, menstrual cycle, and hematological parameters
Author(s) -
Ericsson Magnus,
Bhuiyan Hasanuzzaman,
Yousif Basam,
Lehtihet Mikael,
Ekström Lena
Publication year - 2020
Publication title -
drug testing and analysis
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.065
H-Index - 54
eISSN - 1942-7611
pISSN - 1942-7603
DOI - 10.1002/dta.2953
Subject(s) - menstrual cycle , medicine , biomarker , endocrinology , hormone , hemoglobin , growth hormone , correlation , biology , mathematics , biochemistry , geometry
The intra‐individual stability of growth hormone (GH) biomarkers IGF‐I, P‐III‐NP, calculated GH‐2000 score in relation to growth hormone‐releasing hormone (GHRH) (Somatorelin) administration, menstrual cycle, and hematological parameters were investigated in four men and eight women, respectively. Moreover, the hematological parameters hemoglobin (Hb) and percentage of reticulocyte (RET%) were statistically analyzed in relation to the GH biomarker parameters for the GHRH administration study and the menstrual cycle study. Longitudinal monitoring of IGF‐I and/or GH‐2000 score proved to be a viable approach to detect the GHRH intake in men, as all four participants show values above individually calculated thresholds (calculated as mean ± 3SD from three baseline samples). The intra‐individual variation for IGF‐I, P‐III‐NP, and calculated GH‐2000 score in women, over two consecutive menstrual cycles, was investigated and established to be higher (coefficients variations [CVs] between 12% and 186%) than in men (CVs between 3% and 12%). The GHRH administration did not influence the hematological parameters. A strong positive correlation between Hb and IGF‐I ( R s = 0.73, p < 0.0001) and a borderline weak correlation between RET% and IGF‐I ( R s = 0.28, p = 0.054) were noticed in the women. No correlation for the P‐III‐NP and the hematological parameters was seen for the females in the menstrual cycle study. The results fortify previous studies that longitudinal monitoring of IGF‐I and/or GH‐2000 score may be a promising method to detect doping with GH and GH stimulating agents in men, whereas the large intra‐individual variation noted in women indicates that longitudinal monitoring of these biomarker may be harder to evaluate in women.