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Identification of ex vivo catabolites of peptides with doping potential in equine plasma by HILIC‐HRMS
Author(s) -
Guan Fuyu,
Fay Savannah,
Li Xiaoqing,
You Youwen,
Robinson Mary A.
Publication year - 2020
Publication title -
drug testing and analysis
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.065
H-Index - 54
eISSN - 1942-7611
pISSN - 1942-7603
DOI - 10.1002/dta.2781
Subject(s) - dermorphin , chemistry , peptide , exopeptidase , ex vivo , in vivo , endopeptidase , biochemistry , residue (chemistry) , carboxypeptidase , amino acid , opioid peptide , in vitro , enzyme , opioid , receptor , biology , microbiology and biotechnology
Bioactive peptides pose a great threat to sports integrity. The detection of these peptides is essential for enforcing their prohibition in sports. Identifying the catabolites of these peptides that are formed ex vivo in plasma may improve their detection. In the present study, the stability of 27 bioactive peptides with protection at both termini in equine plasma was examined under different incubation conditions, using HILIC coupled to HRMS. Of the 27 peptides, 13 were stable after incubation at 37°C for 72 hr, but the remaining 14 were less stable. E x vivo catabolites of these 14 peptides were detected using their theoretical masses generated in silico , their appearance was monitored over the time course of incubation, and their identity was verified by their product ion spectra. Catabolites identified for chemotactic peptide, DALDA, dmtDALDA, deltorphins I and II, Hyp 6 ‐dermorphin, Lys 7 ‐dermorphin, and dermorphin analog are novel. A d ‐amino acid residue at position 2 or 1 of a peptide or next to its C‐terminus protected the relevant terminal from degradation by exopeptidases, but such a residue at position 3 did not. A pGlu residue or N‐methylation at the N‐terminus of a peptide did not protect its N‐terminal. Ethylamide at the C‐terminus of a peptide provided the C‐terminal protection from attacks by carboxypeptidases. The C‐terminal Lys amide in DALDA, dmtDALDA, and Lys 7 ‐dermorphin was susceptible to cleavage by plasma enzymes, which is the first report, to the authors’ knowledge. The results from the present study provide insights into the stability of peptides in plasma.