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Rapid inexpensive assay method for verapamil by spectrophotometric sequential injection analysis
Author(s) -
Idris Abubakr M.,
Ibrahim Atta E. E.,
Abulkibash Abdalla M.,
Saleh Tawfik A.,
Ibrahim Kamal E. E.
Publication year - 2011
Publication title -
drug testing and analysis
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.065
H-Index - 54
eISSN - 1942-7611
pISSN - 1942-7603
DOI - 10.1002/dta.277
Subject(s) - chromatography , verapamil , factorial experiment , reagent , spectrophotometry , chemistry , volume (thermodynamics) , dosage form , computer science , calcium , physics , organic chemistry , quantum mechanics , machine learning
Sequential injection analysis (SIA) technique with a miniaturized fibre optic spectrophotometry was exploited to optimize and validate a new method for the assay of verapamil in pharmaceutical formulations. The reduction of acidified permanganate by verapamil was spectrophotometrically detected at 546 nm. The 2 3 full‐factorial design was adopted for screening the effect of conditions controlling the proposed method, and accordingly for the purpose of optimization. The remarkable advantages of the method are high rapidity (sample frequency was 10.6 samples/h), saving in reagents and sample (total consumed volume was 190 µl) and better safety for the environment (total waste production volume was 2140 µl). Additionally, the method was selective in the presence of excipients usually found in tablet and injection formulations. The average of recovery in synthetic samples as well as dosage forms was 98.8–103.0%. The obtained results were realized by the British Pharmacopoeia method and comparable results were obtained. Copyright © 2011 John Wiley & Sons, Ltd.