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A rapid non‐target screening method for determining prohibited substances in human urine using liquid chromatography/high‐resolution tandem mass spectrometry
Author(s) -
Han Boyoung,
Min Hophil,
Jeon Mijin,
Kang Byeori,
Son Junghyun
Publication year - 2019
Publication title -
drug testing and analysis
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.065
H-Index - 54
eISSN - 1942-7611
pISSN - 1942-7603
DOI - 10.1002/dta.2495
Subject(s) - chromatography , mass spectrometry , chemistry , resolution (logic) , tandem mass spectrometry , urine , extraction (chemistry) , liquid chromatography–mass spectrometry , solid phase extraction , detection limit , matrix (chemical analysis) , computer science , artificial intelligence , biochemistry
Target analysis using liquid chromatography–tandem mass spectrometry is applied for rapidly detecting various prohibited doping substances. Frequent modification is required as additional substances are prohibited. We developed and validated a non‐target screening method requiring no further modification because it analyzes the full spectrum of data in fixed m/z ranges. Urine samples were extracted using solid‐phase extraction and analyzed by employing a method that combines full scan and variable data independent acquisition using high‐resolution mass spectrometry; and all prohibited substances in the urine samples were successfully detected using our screening method. The method was validated in terms of specificity (no interferences), recoveries (29%–131%), matrix effects (35%–237%), limites of detection (0.0002–100 ng/mL), and intra‐ and inter‐day precisions (coefficients of variation lower than 25%). The applicability of this method to doping tests was evaluated by analyzing 14 urine samples. As a result, the non‐target screening method is efficient for conducting anti‐doping tests because it can be applied without any further modification to prohibited drugs as well as to unknown targets that can be prohibited in the future.