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Simultaneous determination of iridoid glycosides and flavanoids in Lamionphlomis rotate and its herbal preparation by a simple and rapid capillary zone electrophoresis method
Author(s) -
Lü Wenjuan,
Li Maoxing,
Chen Yonglei,
Chen Hongli,
Chen Xingguo
Publication year - 2012
Publication title -
drug testing and analysis
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.065
H-Index - 54
eISSN - 1942-7611
pISSN - 1942-7603
DOI - 10.1002/dta.248
Subject(s) - chemistry , chromatography , capillary electrophoresis , glycoside , luteolin , iridoid , analyte , apigenin , quercetin , flavonoid , organic chemistry , antioxidant
Iridoid glycosides and flavanoids are two main effective components of Lamiophlomis rotata (Benth.) kudo. However, there is no method for simultaneous analysis of iridoid glycosides and flavanoids in L. rotata and its pharmaceutical preparations. A simple and rapid capillary zone electrophoresis (CZE) method was developed and validated for simultaneous determination of two iridoid glycosides (8‐O‐acetylshanzhiside methylester and 8‐deoxyshanzhiside) and three flavanoids (apigenin, quercetin and luteolin) in L. rotata . Operational variables, such as the voltage, buffer concentration and pH were optimized, the final optimum separation condition was 10 mM sodium tetraborate‐20 mM NaH 2 PO 4 (pH 8.5)‐15% (v/v) methanol, 238 nm UV detection, 18 kV applied voltage. The linearity and the recovery of the proposed method were very satisfactory (correlation coefficients were 0.9994–0.9998 and the recoveries were 94.5–108.8% for the analytes) and the method allowed analytes in real samples to be determined within 9 min. The proposed CZE method can be used for quality control of iridoid glycosides and flavanoids in L. rotata and its herbal preparation. Copyright © 2011 John Wiley & Sons, Ltd.