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Enzyme‐assisted synthesis of the glucuronide conjugate of psilocin, an hallucinogenic component of magic mushrooms
Author(s) -
Shoda Takuji,
Fukuhara Kiyoshi,
Goda Yukihiro,
Okuda Haruhiro
Publication year - 2011
Publication title -
drug testing and analysis
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.065
H-Index - 54
eISSN - 1942-7611
pISSN - 1942-7603
DOI - 10.1002/dta.244
Subject(s) - chemistry , glucuronide , metabolite , chromatography , urine , enzyme , conjugate , stereochemistry , biochemistry , mathematical analysis , mathematics
An enzyme‐assisted synthesis of psilocin glucuronide (PCG), a metabolite excreted in the urine of magic mushroom (MM) users, is described. In the presence of Aroclor 1254 pretreated rat liver microsomes, psilocin and the cofactor UDPGA were incubated for 20 h. Purification by HPLC gave PCG in 19% yield (3.6 mg). The compound structure was characterized by MS and NMR. The milligram amounts of PCG produced by this method will allow the direct identification and quantification of PCG in the urine of MM users. Copyright © 2011 John Wiley & Sons, Ltd.