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Development and validation of a liquid chromatography–tandem mass spectrometry method for the determination of nicotine and its metabolites in placenta and umbilical cord
Author(s) -
PaniaguaGonzález Lucía,
JiménezMorigosa Cristian,
Lendoiro Elena,
Concheiro Marta,
Cruz Angelines,
LópezRivadulla Manuel,
Castro Ana
Publication year - 2018
Publication title -
drug testing and analysis
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.065
H-Index - 54
eISSN - 1942-7611
pISSN - 1942-7603
DOI - 10.1002/dta.2381
Subject(s) - cotinine , chromatography , chemistry , tandem mass spectrometry , nicotine , liquid chromatography–mass spectrometry , solid phase extraction , mass spectrometry , detection limit , umbilical cord , medicine , anatomy
Tobacco exposure during pregnancy is associated with obstetric and fetal complications. We developed and validated a liquid chromatography–tandem mass spectrometry (LC–MS/MS) method to determine nicotine, cotinine, and hydroxycotinine (OH‐cotinine) in placenta (PL) and umbilical cord (UC). Specimens were homogenized in water, followed by solid‐phase extraction. Chromatographic separation was performed using an Atlantis® HILIC Silica column. Detection was accomplished in electrospray in positive mode. Method validation included: linearity (5 to 1000 ng/g), accuracy (86.9 to 105.2% of target concentration in PL, and 89.1 to 105.0% in UC), imprecision (6.8 to 11.8% in PL, and 7.6 to 12.2% in UC), limits of detection (2 ng/g for cotinine and OH‐cotinine, and 5 ng/g for nicotine) and quantification (5 ng/g), selectivity (no endogenous or exogenous interferences), matrix effect (−34.1 to −84.5% in PL, %CV = 9.1–24.0%; −18.9 to −84.7% in UC, %CV = 10.2–23.9%), extraction efficiency (60.7 to 131.5% in PL, and 64.1 to 134.2% in UC), and stability 72 h in the autosampler (<11.5% loss in PL, and < 13% loss in UC). The method was applied to 14 PL and UC specimens from tobacco users during pregnancy. Cotinine (6.8–312.2 ng/g in PL; 6.7–342.3 ng/g in UC) was the predominant analyte, followed by OH‐cotinine (