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Transcriptomic biomarkers of altered erythropoiesis to detect autologous blood transfusion
Author(s) -
Salamin Olivier,
Mignot Jonathan,
Kuuranne Tiia,
Saugy Martial,
Leuenberger Nicolas
Publication year - 2018
Publication title -
drug testing and analysis
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.065
H-Index - 54
eISSN - 1942-7611
pISSN - 1942-7603
DOI - 10.1002/dta.2240
Subject(s) - reticulocyte , erythropoiesis , transcriptome , gene , bone marrow , biology , candidate gene , blood transfusion , gene expression , microbiology and biotechnology , immunology , andrology , medicine , messenger rna , anemia , genetics
Autologous blood transfusion is a powerful means of improving performance and remains one of the most challenging methods to detect. Recent investigations have identified 3 candidate reticulocytes genes whose expression was significantly influenced by blood transfusion. Using quantitative reverse transcription polymerase chain reaction as an alternative quantitative method, the present study supports that delta‐aminolevulinate synthase 2 ( ALAS2), carbonic anhydrase (CA1), and solute carrier family 4 member 1 ( SLC4A1) genes are down‐regulated post‐transfusion. The expression of these genes exhibited stronger correlation with immature reticulocyte fraction than with reticulocytes percentage. Moreover, the repression of reticulocytes' gene expression was more pronounced than the diminution of immature reticulocyte fraction and reticulocyte percentage following blood transfusion. It suggests that the 3 candidate genes are reliable predictors of bone marrow's response to blood transfusion and that they represent potential biomarkers for the detection of this method prohibited in sports.