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Analysis of RBC‐microparticles in stored whole blood bags – a promising marker to detect blood doping in sports?
Author(s) -
Voss Sven Christian,
Jaganjac Morana,
AlThani Amna Mohamed,
Grivel JeanCharles,
Raynaud Christophe Michel,
AlJaber Hind,
AlMenhali Afnan Saleh,
Merenkov Zeyed Ahmad,
Alsayrafi Mohammed,
Latiff Aishah,
Georgakopoulos Costas
Publication year - 2017
Publication title -
drug testing and analysis
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.065
H-Index - 54
eISSN - 1942-7611
pISSN - 1942-7603
DOI - 10.1002/dta.2212
Subject(s) - whole blood , biomarker , platelet , red blood cell , medicine , blood transfusion , platelet poor plasma , blood plasma , blood cell , chemistry , platelet rich plasma , biochemistry
Blood doping in sports is prohibited by the World Anti‐Doping Agency (WADA). To find a possible biomarker for the detection of blood doping, we investigated the changes in blood stored in CPDA‐1 blood bags of eight healthy subjects who donated one unit of blood. Aliquots were taken on days 0, 14, and 35. Platelet‐free plasma was prepared and stored at −80°C until analysis on a flow cytometer dedicated for the analysis of microparticles (MPs). Changes in the number of red blood cell (RBC) ‐MPs were highly significant ( p < 0.0001) with a mean of 219 (10^3/μL) on day 0 changing to 23 120 (10^3/μL) on day 14 and 29 310 (10^3/μL) on day 35. We conclude that RBC‐MPs seem to be a promising biomarker for doping control but confirmation by a transfusion study is necessary.