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Characterization of hallucinogenic phenethylamines using high‐resolution mass spectrometry for non‐targeted screening purposes
Author(s) -
Pasin Daniel,
Cawley Adam,
Bidny Sergei,
Fu Shanlin
Publication year - 2017
Publication title -
drug testing and analysis
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.065
H-Index - 54
eISSN - 1942-7611
pISSN - 1942-7603
DOI - 10.1002/dta.2171
Subject(s) - phenethylamines , designer drug , mass spectrometry , chemistry , dissociation (chemistry) , hallucinogen , chromatography , stereochemistry , medicinal chemistry , pharmacology , organic chemistry , medicine , drug
Hallucinogenic phenethylamines such as 2,5‐dimethoxyphenethylamines (2C–X) and their N ‐(2‐methoxybenzyl) derivatives (25X–NBOMe) have seen an increase in novel analogues in recent years. These rapidly changing analogues make it difficult for laboratories to rely on traditional targeted screening methods to detect unknown new psychoactive substances (NPS). In this study, twelve 2C–X, six 2,5‐dimethoxyamphetamines (DOX), and fourteen 25X–NBOMe derivatives, including two deuterated derivatives (2C–B‐ d 6 and 25I–NBOMe‐ d 9 ), were analyzed using ultra‐performance liquid chromatography coupled with quadrupole time‐of‐flight mass spectrometry (UPLC‐QTOF‐MS). Collision‐induced dissociation (CID) experiments were performed using collision energies set at 10, 20, and 40 eV. For 2C–X and DOX derivatives, common losses were observed including neutral and radical losses such as NH 3 (17.0265 Da), •CH 6 N (32.0500 Da), C 2 H 7 N (45.0578 Da) and C 2 H 9 N (47.0735 Da). 2C–X derivatives displayed common product ions at m/z 164.0837 ([C 10 H 12 O 2 ] +• ), 149.0603 ([C 9 H 9 O 2 ] + ), and 134.0732 ([C 9 H 10 O] +• ) while DOX derivatives had common product ions at m/z 178.0994 ([C 11 H 14 O 2 ] +• ), 163.0754 ([C 10 H 11 O 2 ] + ), 147.0804 ([C 10 H 11 O] + ), and 135.0810 ([C 9 H 11 O] + ). 25X–NBOMe had characteristic product ions at m/z 121.0654 ([C 8 H 9 O] + ) and 91.0548 ([C 7 H 7 ] + ) with minor common losses corresponding to 2‐methylanisole (C 8 H 10 O, 122.0732 Da), 2‐methoxybenzylamine (C 8 H 11 NO, 137.0847 Da), and •C 9 H 14 NO (152.1074 Da). Novel analogues of the selected classes can be detected by applying neutral loss filters (NLFs) and extracting the common product ions. Copyright © 2017 John Wiley & Sons, Ltd.