Premium
Doping control analysis of anabolic steroids in equine urine by gas chromatography‐tandem mass spectrometry
Author(s) -
Wong April S. Y.,
Leung Gary N. W.,
Leung David K. K.,
Wan Terence S. M.
Publication year - 2017
Publication title -
drug testing and analysis
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.065
H-Index - 54
eISSN - 1942-7611
pISSN - 1942-7603
DOI - 10.1002/dta.2090
Subject(s) - chemistry , chromatography , mass spectrometry , gas chromatography , tandem mass spectrometry , gas chromatography–mass spectrometry , triple quadrupole mass spectrometer , urine , gas chromatography/tandem mass spectrometry , analyte , electrospray ionization , selected reaction monitoring , biochemistry
Anabolic steroids are banned substances in equine sports. Gas chromatography‐mass spectrometry (GC‐MS) has been the traditional technique for doping control analysis of anabolic steroids in biological samples. Although liquid chromatography‐mass spectrometry (LC/MS) has become an important technique in doping control, the detection of saturated hydroxysteroids by LC‐MS remains a problem due to their low ionization efficiency under electrospray. The recent development in fast‐scanning gas‐chromatography‐triple‐quadrupole mass spectrometry (GC‐MS/MS) has provided a better alternative with a significant reduction in chemical noise by means of selective reaction monitoring. Herein, we present a sensitive and selective method for the screening of over 50 anabolic steroids in equine urine using gas chromatography‐tandem mass spectrometry (GC‐MS/MS). Copyright © 2016 John Wiley & Sons, Ltd.