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Rapid determination of nine barbiturates in human whole blood by liquid chromatography‐tandem mass spectrometry
Author(s) -
Zhang Xinyu,
Lin Zebin,
Li Jiaolun,
Huang Zhibin,
Rao Yulan,
Liang Hao,
Yan Jie,
Zheng Feng
Publication year - 2017
Publication title -
drug testing and analysis
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.065
H-Index - 54
eISSN - 1942-7611
pISSN - 1942-7603
DOI - 10.1002/dta.2002
Subject(s) - secobarbital , chromatography , chemistry , barbital , amobarbital , pentobarbital , barbiturate , hexobarbital , extraction (chemistry) , detection limit , pharmacology , medicine , biochemistry , neuroscience , epilepsy , biology , enzyme , microsome
A rapid, simple and sensitive liquid chromatography‐tandem mass spectrometry (LC‐MS/MS) method was developed for the qualitative and quantitative analysis of nine barbiturates (barbital, phenobarbital, pentobarbital, amobarbital, secobarbital, thiopental, butalbital, butabarbital, and hexobarbital) in human whole blood. Barbiturates were extracted from 100 μL of human whole blood samples using a simple liquid‐liquid extraction (LLE) procedure, and detected by LC‐MS/MS. An UPLC C18 (2.1 mm × 100 mm, 1.7 µm) column was used at 40 °C for the separation and acetonitrile/water system was used as the mobile phase with gradient elution. This method showed excellent accuracy (86–111%) and precision (relative standard deviation <15%). The limits of detection (LODs) were 0.2 ng/mL for barbital and secobarbital and 0.5 ng/mL for the other barbiturates. The linearity ranged from 2 ng/mL to 2000 ng/mL, with r 2 > 0.99 over the range. This method achieved the separation and detection of pentobarbital and amobarbital at the same time in a convenient way. Moreover, it was both simple and sensitive for the determination of nine most commonly used barbiturate drugs, which was meaningful in the field of clinical and forensic toxicology. Copyright © 2016 John Wiley & Sons, Ltd.