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A fast preparative method for detection of recombinant erythropoietin in blood samples
Author(s) -
Lasne Françoise,
Martin Laurent,
Martin Jean Antoine
Publication year - 2010
Publication title -
drug testing and analysis
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.065
H-Index - 54
eISSN - 1942-7611
pISSN - 1942-7603
DOI - 10.1002/dta.171
Subject(s) - erythropoietin , urine , isoelectric focusing , chromatography , chemistry , recombinant dna , darbepoetin alfa , medicine , biochemistry , enzyme , gene
Isoelectric focusing is the basis of anti‐doping control of recombinant erythropoietin in urine. A convenient preparative method is proposed for screening of Continuous Erythropoietin Receptor Activator (CERA) and Darbepoetin alfa in serum or plasma. This method makes isoelectric focusing as easy to use in blood as in urine. In comparison with urine, EPO analyses in blood appear beneficial in terms of sensitivity, stability during physical exercise, and prevention of sample adulteration at collection.