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Rapid determination of memantine in human plasma by using nanoring carboxyl‐functionalized paramagnetic molecularly imprinted polymer d‐ μ ‐SPE and UFLC‐MS/MS
Author(s) -
Qiu HaiWen,
Xia Lei,
Gong LiMin,
Ruan LieMin,
Zhao YongGang
Publication year - 2015
Publication title -
drug testing and analysis
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.065
H-Index - 54
eISSN - 1942-7611
pISSN - 1942-7603
DOI - 10.1002/dta.1706
Subject(s) - molecularly imprinted polymer , chromatography , nanoring , chemistry , solid phase extraction , divinylbenzene , suspension polymerization , extraction (chemistry) , polymerization , polymer , materials science , styrene , organic chemistry , copolymer , selectivity , catalysis , optoelectronics
A novel, simple, and sensitive method based on the use of dispersive micro‐solid‐phase extraction (d‐ μ ‐SPE) procedure combined with ultra‐fast liquid chromatography‐tandem quadrupole mass spectrometry (UFLC‐MS/MS) for the determination of memantine (ME) was developed and validated over the linearity range 0.05–10.0 µg/L with 100 μL of human plasma using memantine‐D6 (ME‐D6) as the internal standard. The novel nanoring carboxyl‐functionalized paramagnetic molecularly imprinted polymer (NR‐CF‐Mag‐MIP) was synthesized by ultrasound‐assisted suspension polymerization, using ME as a template molecule, methacrylic acid as a functional monomer, and divinylbenzene as a cross‐linking agent. The NR‐CF‐Mag‐MIP was used as the d‐ μ ‐SPE sorbent to extract ME from human plasma samples. The obtained results demonstrated the higher extraction capacity of NR‐CF‐Mag‐MIP with recoveries between 97.6 and 101%. The limits of quantification (LOQs) for ME was 0.015 µg/L. Validation results on linearity, specificity, accuracy, precision, and stability, as well as on application to the analysis of samples taken up to 480 h after oral administration of 20 mg (two 10 mg capsules) of ME in healthy volunteers demonstrated the applicability to bioequivalence studies. Copyright © 2014 John Wiley & Sons, Ltd.