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Impact of oral fluid collection device on cannabinoid stability following smoked cannabis
Author(s) -
Anizan Sébastien,
Bergamaschi Mateus M.,
Barnes Allan J.,
Milman Garry,
Desrosiers Nathalie,
Lee Dayong,
Gorelick David A.,
Huestis Marilyn A.
Publication year - 2015
Publication title -
drug testing and analysis
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.065
H-Index - 54
eISSN - 1942-7611
pISSN - 1942-7603
DOI - 10.1002/dta.1688
Subject(s) - cannabis , cannabinoid , marijuana smoking , dronabinol , street drugs , tetrahydrocannabinol , medicine , pharmacology , psychiatry , substance use , drug , receptor , polysubstance dependence
Evaluation of cannabinoid stability in authentic oral fluid (OF) is critical, as most OF stability studies employed fortified or synthetic OF. Participants (n = 16) smoked a 6.8% delta‐9‐tetrahydrocannabinol (THC) cigarette, and baseline concentrations of THC, 11‐nor‐9‐carboxy‐THC (THCCOOH), cannabidiol (CBD), and cannabinol (CBN) were determined within 24 h in 16 separate pooled samples (collected 1 h before to 10.5 or 13 h after smoking). OF was collected with the StatSure Saliva Sampler™ and Oral‐Eze® devices. Oral‐Eze samples were re‐analyzed after room temperature (RT) storage for 1 week, and for both devices after 4 °C for 1 and 4 weeks, and −20 °C for 4 and 24 weeks. Concentrations ±20% from initial concentrations were considered stable. With the StatSure device, all cannabinoids were within 80–120% median %baseline for all storage conditions. Individual THC, CBD, CBN and THCCOOH pool concentrations were stable in 100%, 100%, 80–94% and >85%, respectively, across storage conditions. With the Oral‐Eze device, at RT or refrigerated storage (for 1 and 4 weeks), THC, CBD and THCCOOH were stable in 94–100%, 78–89%, and 93–100% of samples, respectively, while CBN concentrations were 53–79% stable. However, after 24 weeks at −20 °C, stability decreased, especially for CBD, with a median of 56% stability. Overall, the collection devices’ elution/stabilizing buffers provided good stability for OF cannabinoids, with the exception of the more labile CBN. To ensure OF cannabinoid concentration accuracy, these data suggest analysis within 4 weeks at 4 °C storage for Oral‐Eze collection and within 4 weeks at 4 °C or 24 weeks at −20 °C for StatSure collection. Published 2014. This article is a U.S. Government work and is in the public domain in the USA.