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UPLC‐MS/MS determination of ephedrine, methylephedrine, amygdalin and glycyrrhizic acid in Beagle plasma and its application to a pharmacokinetic study after oral administration of Ma Huang Tang
Author(s) -
Yan Tianhua,
Fu Qiang,
Wang Jing,
Ma Shiping
Publication year - 2015
Publication title -
drug testing and analysis
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.065
H-Index - 54
eISSN - 1942-7611
pISSN - 1942-7603
DOI - 10.1002/dta.1635
Subject(s) - amygdalin , chemistry , chromatography , ephedrine , formic acid , selected reaction monitoring , beagle , mass spectrometry , electrospray ionization , extraction (chemistry) , pharmacokinetics , high performance liquid chromatography , tandem mass spectrometry , pharmacology , medicine , alternative medicine , pathology
An ultra performance liquid chromatography‐mass spectrometric (UPLC‐MS) method was developed to investigate the pharmacokinetic properties of ephedrine, methylephedrine, amygdalin, and glycyrrhizic acid after oral gavage of Ma Huang Tang (MHT) in Beagles. Beagle plasma samples were pretreated using liquid‐liquid extraction, and chromatographic separation was performed on a C18 column using a linear gradient of water‐formic acid mixture (0.1%). The pharmacokinetic parameters of ephedrine, methylephedrine, amygdalin, and glycyrrhizic acid from MHT in Beagles were quantitatively determined by UPLC with tandem mass detector. The qualitative detection of the four compounds was accomplished by selected ion monitoring in negative/positive ion modes electrospray ionization‐mass spectrometry (ESI‐MS). Detection was based on multiple reaction monitoring with the precursor‐to‐product ion transitions m/z 166.096–116.983 (ephedrine), m/z 179.034–146.087 (methylephedrine), m/z 456.351–323.074 (amygdalin), and m/z 821.606–351.062 (glycyrrhizic acid). The selectivity, sensitivity, linearity, accuracy, precision, extraction recovery, ion suppression, and stability were within the acceptable ranges. The method described was successfully applied to reveal the pharmacokinetic properties of ephedrine, methylephedrine, amygdalin, and glycyrrhizic acid after oral gavage of MHT in Beagles. Copyright © 2014 John Wiley & Sons, Ltd.