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Spectrophotometric determination of penicillins in pure and pharmaceutical formulations using Folin‐Ciocalteu reagent
Author(s) -
Singh Dhruv K.,
Maheshwari Gunjan
Publication year - 2010
Publication title -
drug testing and analysis
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.065
H-Index - 54
eISSN - 1942-7611
pISSN - 1942-7603
DOI - 10.1002/dta.157
Subject(s) - reagent , ampicillin , chemistry , amoxicillin , chromogenic , molar absorptivity , chromatography , spectrophotometry , tungstate , dosage form , analyte , antibiotics , organic chemistry , biochemistry , physics , optics
A new combination of time, temperature, and alkali is described for the spectrophotometric determination of amoxicillin and ampicillin using Folin‐Ciocalteu reagent. The method is based on the development of blue‐coloured product due to the reduction of tungstate and/or molybdate in Folin‐Ciocalteu reagent by amoxicillin and ampicillin in alkaline medium. The chromogenic reaction has λ max at 720 and 740 nm with molar absorptivity 1.6295 × 10 4 and 0.1085 × 10 4 l mol −1 cm −1 in the Beer's Law range 2–10 µg mL −1 and 10–70 µg mL −1 for amoxicillin and ampicillin, respectively. The method is reproducible, quick, inexpensive, and particularly helpful in determining the drug content in commercial dosage forms. Copyright © 2010 John Wiley & Sons, Ltd.