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Development and validation of fixed‐time method for the determination of isoxsuprine hydrochloride in commercial dosages forms
Author(s) -
Rahman Nafisur,
Afaq Nasheed
Publication year - 2010
Publication title -
drug testing and analysis
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.065
H-Index - 54
eISSN - 1942-7611
pISSN - 1942-7603
DOI - 10.1002/dta.153
Subject(s) - calibration curve , absorbance , hydrochloride , chemistry , cerium , molar absorptivity , chromatography , hydroxylamine hydrochloride , detection limit , organic chemistry , physics , optics
The main aim of this work was to develop a kinetic spectrophotometric method for the quantitative analysis of isoxsuprine hydrochloride in commercial tablets. The method is based on the reaction of isoxsuprine hydrochloride (ISx) with hydroxylamine hydrochloride and ammonium cerium (IV) nitrate in sulphuric acid medium at room temperature which resulted in the formation of yellow‐coloured product peaking at 380 nm. The reaction is followed spectrophotometrically by measuring the absorbance as a function of time. Fixed time method (ΔA = A 4 –A 2 , where A 2 and A 4 refer to absorbance measurements taken at 2 and 4 min, respectively) was adopted for constructing the calibration curve which was found to be linear over the concentration range of 30–80 µgmL −1 with molar absorptivity of 5.95 × 10 3 L mol −1 cm −1 . The method has been applied successfully to the determination of isoxsuprine hydrochloride in tablets. Statistical comparison (point and interval hypothesis tests) of the results showed that there is no significant difference between the proposed method and reference method. Copyright © 2010 John Wiley & Sons, Ltd.