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Optimization and validation of CEDIA drugs of abuse immunoassay tests in serum and urine on an Olympus AU 400
Author(s) -
Musshoff F.,
Wolters T.,
Lott S.,
Ippisch J.,
Gradl S.,
Madea B.
Publication year - 2013
Publication title -
drug testing and analysis
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.065
H-Index - 54
eISSN - 1942-7611
pISSN - 1942-7603
DOI - 10.1002/dta.1454
Subject(s) - urine , immunoassay , chromatography , drugs of abuse , chemistry , receiver operating characteristic , mass spectrometry , detection limit , spectrum analyzer , poison control , drug , medicine , pharmacology , medical emergency , computer science , immunology , biochemistry , antibody , telecommunications
A preliminary initial cloned enzyme donor immunoassay (CEDIA) was optimized for serum and urine drug testing with respect to the German per se limits for driving under the influence of drugs (serum) and lowered cut‐offs in cases of driving licence re‐granting (urine). The tests were performed on an Olympus AU 400 auto analyzer. Validation revealed sensitivities between 93% and 100% based on comparison with data from gas or liquid chromatography coupled with mass spectrometry. Even if specificity ranged between 83% and 98 %, the tests can be considered useful for forensic purposes. Receiver operating characteristic (ROC) curves, Youden indices, as well as positive and negative predictive values are presented. Copyright © 2013 John Wiley & Sons, Ltd.