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Application of high‐throughput IEF‐PAGE for EPO‐doping testing
Author(s) -
Reichel Christian
Publication year - 2012
Publication title -
drug testing and analysis
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.065
H-Index - 54
eISSN - 1942-7611
pISSN - 1942-7603
DOI - 10.1002/dta.1411
Subject(s) - throughput , computer science , operating system , wireless
Since its first publication in 2000, the isoelectric focusing (IEF) method of erythropoietin (EPO) used in doping control has been considered a procedure with relatively small sample number capacity. To overcome this limitation, a variation of the current protocol was evaluated, which uses double‐sized gels with 48–120 wells plus three electrodes and hence multiplies the capacity of the electrophoretic chamber. With this modification up to 120 samples and standards can be run on a single gel – thus, making IEF‐PAGE of EPO a high‐throughput method. The protocol is ideally suited for large‐scale screening purposes. Copyright © 2012 John Wiley & Sons, Ltd.