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Synthesis and characterization of the N‐terminal acetylated 17‐23 fragment of thymosin beta 4 identified in TB‐500, a product suspected to possess doping potential
Author(s) -
Esposito Simone,
Deventer Koen,
Goeman Jan,
Van der Eycken Johan,
Van Eenoo Peter
Publication year - 2012
Publication title -
drug testing and analysis
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.065
H-Index - 54
eISSN - 1942-7611
pISSN - 1942-7603
DOI - 10.1002/dta.1402
Subject(s) - mass spectrometry , chromatography , thymosin , chemistry , acetylation , peptide , orbitrap , high performance liquid chromatography , resolution (logic) , biochemistry , artificial intelligence , computer science , gene
The formulation TB‐500 is suspected to be used as doping agent in sport. This work describes the detection and the identification of the N‐terminal acetylated 17‐23 fragment of human thymosin beta 4 (Ac‐LKKTETQ) in TB‐500 by means of high‐performance liquid chromatography/high resolution mass spectrometry using an Orbitrap Exactive benchtop mass spectrometer. Ac‐LKKTETQ was also synthesized by solid‐phase peptide synthesis, and an analytical strategy for detection in plasma and urine by high‐performance liquid chromatography/low resolution triple‐quadrupole mass spectrometry was suggested.