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Electrochemical study of spiramycin and its determination in pharmaceutical preparation
Author(s) -
Youssef Rasha M.,
Maher Hadir M.
Publication year - 2010
Publication title -
drug testing and analysis
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.065
H-Index - 54
eISSN - 1942-7611
pISSN - 1942-7603
DOI - 10.1002/dta.137
Subject(s) - spiramycin , polarography , chemistry , hanging mercury drop electrode , dropping mercury electrode , electrochemistry , supporting electrolyte , detection limit , electrode , chromatography , analytical chemistry (journal) , nuclear chemistry , voltammetry , inorganic chemistry , antibiotics , biochemistry , erythromycin
Spiramycin (SPY) is a medium‐spectrum antibiotic with high effectiveness against Gram‐positive bacteria. The voltammetric behaviour of spiramycin was studied using differential pulse polarography (DPP) and square wave polarography (SWP). The drug in Britton‐Robinson buffer (pH 11.5) is reduced at − 1.45 V, giving rise to a well‐defined cathodic peak using hanging mercury drop electrode (HMDE) versus Ag/AgCl electrode. This peak is attributed to the reduction of the aldehyde group. The results proved that the reduction of SPY is an irreversible diffusion‐controlled process. The diffusion current‐concentration relationship was shown to be rectilinear over the range of 20–80 and 0.8–80 µg ml −1 using DPP and SWP modes, respectively, with detection limit of 8.5 µg ml −1 (1.01 × 10 −5 M) and 0.46 µg ml −1 (5.46 × 10 −7 M) for DPP and SWP modes, respectively. A mechanism is postulated for the reduction of SPY. The proposed techniques were successfully applied to the determination of the studied compound either in pure form or in its formulation. Copyright © 2010 John Wiley & Sons, Ltd.