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Evaluation of a multi‐class, multi‐residue liquid chromatography – tandem mass spectrometry method for analysis of 120 veterinary drugs in bovine kidney
Author(s) -
Schneider Marilyn J.,
Lehotay Steven J.,
Lightfield Alan R.
Publication year - 2012
Publication title -
drug testing and analysis
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.065
H-Index - 54
eISSN - 1942-7611
pISSN - 1942-7603
DOI - 10.1002/dta.1359
Subject(s) - chromatography , analyte , chemistry , veterinary drug , liquid chromatography–mass spectrometry , tandem mass spectrometry , veterinary drugs , residue (chemistry) , mass spectrometry , matrix (chemical analysis) , detection limit , medicine , biochemistry , veterinary medicine
Traditionally, regulatory monitoring of veterinary drug residues in food animal tissues involves the use of several single‐class methods to cover a wide analytical scope. Multi‐class, multi‐residue methods (MMMs) of analysis tend to provide greater overall laboratory efficiency than the use of multiple methods, and liquid chromatography–tandem mass spectrometry (LC‐MS/MS) of targeted drug analytes usually provides exceptional performance even for complicated sample extracts. In this work, an LC‐MS/MS method was optimized and validated in a test of 120 drug analytes from 11 different classes in bovine kidney. The method used 10 ml of 4/1 acetonitrile/water for extraction of 2 g samples and cleanup with hexane partitioning. Quantitative and qualitative performance was assessed for the analytes at fortification levels of 10, 50, 100, and 200 ng/g. With the method, 66 drugs gave 70–120% recovery with ≤20% RSD at all levels over the course of 3 days. At the 200 ng/g level, 89 drugs met these same standards. Limits of detection were ≤10 ng/g for 109 of the analytes in the kidney matrix in validation experiments. Qualitatively, MS/MS identification criteria were set that ion ratios occur within ±10% (absolute value) from those of the analyte reference standards. At the 10 ng/g level, 57% of the drugs met the identification criteria, which improved to 84% at the 200 ng/g level. The method serves as an efficient and useful additional option among the current monitoring methods available. Published 2012. This article is a U.S. Government work and is in the public domain of the USA.