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A novel flow‐injection chemiluminescence method for determination of andrographolide in andrographis tablets
Author(s) -
Jiang Zejun,
Hao Zaibin,
Wu Qiong,
Li Yang,
Liu Hongyan,
Yan Li
Publication year - 2013
Publication title -
drug testing and analysis
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.065
H-Index - 54
eISSN - 1942-7611
pISSN - 1942-7603
DOI - 10.1002/dta.1346
Subject(s) - andrographolide , chemiluminescence , calibration curve , detection limit , andrographis paniculata , chromatography , relative standard deviation , chemistry , linear range , calibration , linear relationship , analytical chemistry (journal) , mathematics , medicine , biochemistry , statistics , alternative medicine , pathology
A novel method for determination of andrographolide using flow‐injection chemiluminescence (FI‐CL) analysis is described in this paper. The chemiluminescence intensity of the solution was enhanced proportionally while the concentration of andrographolide increased. Under the selected experimental conditions, the calibration curve of andrographolide was linear within the range of 0.2 to 35.0 µg mL −1 with a linear equation of ΔI = 23.391x (µg mL −1 ) + 34.191, R 2 = 0.9965. The detection limit (3σ) was 7.42 × 10 −2 µg mL −1 . At the case of continuous determination of andrographolide, the relative standard deviation (RSD, n = 11) was less than 1.82%. The method has been successfully applied to the determination of andrographis tablets with satisfactory results. Copyright © 2012 John Wiley & Sons, Ltd.