A rapid and most sensitive liquid chromatography/tandem mass spectrometry method for simultaneous determination of alverine and its major metabolite, para hydroxy alverine, in human plasma: application to a pharmacokinetic and bioequivalence study | Zendy

Ghosh Chinmoy | Zendy; Jha Vijay | Zendy; Ahir Ramesh | Zendy; Shah Sujal | Zendy; Shinde C.P. | Zendy; Chakraborty Bhaswat S. | Zendy

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A rapid and most sensitive liquid chromatography/tandem mass spectrometry method for simultaneous determination of alverine and its major metabolite, para hydroxy alverine, in human plasma: application to a pharmacokinetic and bioequivalence study

Author(s) -

Ghosh Chinmoy,

Jha Vijay,

Ahir Ramesh,

Shah Sujal,

Shinde C.P.,

Chakraborty Bhaswat S.

Publication year - 2010

Publication title -

drug testing and analysis

Language(s) - English

Resource type - Journals

SCImago Journal Rank - 1.065

H-Index - 54

eISSN - 1942-7611

pISSN - 1942-7603

DOI - 10.1002/dta.130

Subject(s) - chromatography , chemistry , pharmacokinetics , metabolite , electrospray ionization , bioequivalence , selected reaction monitoring , extraction (chemistry) , mass spectrometry , tandem mass spectrometry , solid phase extraction , calibration curve , active metabolite , electrospray , detection limit , pharmacology , medicine , biochemistry

A rapid and highly sensitive method for the determination of alverine (ALV) and its metabolite, para hydroxy alverine (PHA), in human plasma using LC‐MS/MS in positive ion electrospray ionization (ESI) in multiple reactions monitoring (MRM) mode was developed and validated. The procedure involves a simple solid phase extraction (SPE). Chromatographic separation was carried out on a Hypersil GOLD C 18 column (50 mm × 4.6 mm, 5 µm) with an isocratic mobile phase and a total run time of 1.5 min. The standard calibration curves showed excellent linearity within the range of 0.060–10.051 ng/mL for ALV and 0.059–10.017 ng/mL for PHA (r ≥ 0.990). This method was successfully applied to a pharmacokinetic study after oral administration of alverine citrate 120 mg capsule in Indian healthy male volunteers. Copyright © 2010 John Wiley & Sons, Ltd.

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