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A validated stability‐indicating gas chromatography method for determination of divalproex sodium impurities in pharmaceutical preparation
Author(s) -
Subasranjan Acharya,
Suresh Pulluru,
Srinivasulu C.,
Hemant Raoutray
Publication year - 2010
Publication title -
drug testing and analysis
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.065
H-Index - 54
eISSN - 1942-7611
pISSN - 1942-7603
DOI - 10.1002/dta.117
Subject(s) - chromatography , impurity , gas chromatography , chemistry , divalproex , sodium , medicine , organic chemistry , bipolar disorder , lithium (medication) , mania
A stability‐indicating gas chromatography (GC) method has been developed and validated for the quantitative determination of divalproex sodium impurities in pharmaceutical preparation. A technique has been developed whereby the peak purity of a compound with poor UV detection can be determined using a gas chromatograph coupled with a mass spectrometer. The drug products were subjected to hydrolysis, oxidation, photolysis, and heat to apply stress conditions. The stability‐indicating nature of the method has been proven by establishing peak purity of all stressed samples. The chromatographic separation was performed on a fused silica capillary (Quadrex‐FFAP, 30 meter, 0.32 mm and 1 µm film thickness) column. The method validation results indicate that the method is specific, accurate, linear, reproducible, rugged, and robust. The effectiveness of the technique was demonstrated with stability sample analysis of divalproex sodium in its pharmaceutical preparation. Copyright © 2010 John Wiley & Sons, Ltd.