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Spectrophotometric determination of isopropamide Iodide and trifluoperazine hydrochloride in presence of trifluoperazine oxidative degradate
Author(s) -
Abbas Samah S.,
Zaazaa Hala E.,
Abdelkawy M.,
Abdelrahman Maha M.
Publication year - 2010
Publication title -
drug testing and analysis
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.065
H-Index - 54
eISSN - 1942-7611
pISSN - 1942-7603
DOI - 10.1002/dta.113
Subject(s) - trifluoperazine , chemistry , iodide , derivative (finance) , analytical chemistry (journal) , nuclear chemistry , chromatography , inorganic chemistry , organic chemistry , calmodulin , financial economics , economics , calcium
Four sensitive, selective and precise stability indicating methods for the determination of isopropamide iodide (ISO) and trifluoperazine hydrochloride (TPZ) in their binary mixture and in presence of trifluoperazine oxidative degradate (OXD). Method A is a derivative spectrophotometric one, where ISO was determined by first derivative (D 1 ) at 226.4 nm while TPZ was determined by second derivative (D 2 ) at 270.2 nm. Method B is the first derivative of the ratio spectra (DD 1 ) spectrophotometric method, ISO can be determined by measuring the peak amplitude at 227.4 nm using 5 µg mL −1 of OXD as a divisor, while TPZ can be determined by measuring the peak amplitude at 249.2 and 261.4 nm using 15 µg mL −1 of ISO as a divisor. Method C is the isoabsorptive spectrophotometric method. This method allows determination of ISO and TPZ in their binary mixture by measuring total concentration of ISO and TPZ at their isoabsorptive point at λ 229.8 nm (Aiso 1 ) while TPZ concentration alone can be determined at λ max 311.2 nm, then ISO concentration can be determined by subtraction. On the same basis TPZ can be determined in presence of ISO and OXD, where OXD concentration alone was determined by measuring the peak amplitude at λ 281.6 and λ 309.4 nm while total concentration of TPZ and OXD was determined at their isoabsorptive points at (Aiso 2 = 270.2 nm), (Aiso 3 = 310.6 nm) and (Aiso 4 = 331.8 nm) then TPZ concentration was determined by subtraction. Method D is the multivariate calibration techniques [the classical least squares (CLS), principal component regression (PCR) and partial least squares (PLS)], using the information contained in the absorption spectra of ISO, TPZ and OXD mixtures. The selectivity of the proposed methods was checked using laboratory prepared mixtures. The proposed methods have been successfully applied to the analysis of ISO and TPZ in pharmaceutical dosage form without interference from other dosage form additives and the results were statistically compared with the reported method. Copyright © 2010 John Wiley & Sons, Ltd.