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An application of Ag(III) complex chemiluminescence system for the determination of enoxacin in capsule and biological fluid
Author(s) -
Chen Peiyun,
Sun Hanwen
Publication year - 2010
Publication title -
drug testing and analysis
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.065
H-Index - 54
eISSN - 1942-7611
pISSN - 1942-7603
DOI - 10.1002/dta.104
Subject(s) - enoxacin , chemiluminescence , chemistry , capsule , chromatography , biological fluids , biology , biochemistry , antibiotics , botany , norfloxacin , ciprofloxacin
Ag(III) complex chemiluminescence (CL) system was applied for the determination of enoxacin (ENX). The CL conditions of [Ag(HIO 6 ) 2 ] 5− H 2 SO 4 ENX systems without any luminescence reagent were investigated and optimized. Under the optimized conditions, the CL intensity was proportional to the concentration of ENX in the range from 6.6 × 10 −5 to 3.3 × 10 −3 g/L. The limit of detection ( s / n = 3) was 2.0 × 10 −5 g/L. The recovery of ENX from the spiked pharmaceutical preparations was in the range of 82.9–108% with a relative standard deviation of 1.9–3.0%. For spiked serum and urine samples the recovery of ENX was in the range of 83.7–110% with a relative standard deviation of 1.1–2.8%. The proposed method was applied successfully to the determination of the drug in capsule, serum and urine samples. Copyright © 2010 John Wiley & Sons, Ltd.