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Wnt and Shh signals regulate neural stem cell proliferation and differentiation in the optic tectum of adult zebrafish
Author(s) -
Shitasako Shiori,
Ito Yoko,
Ito Ryoichi,
Ueda Yuto,
Shimizu Yuki,
Ohshima Toshio
Publication year - 2017
Publication title -
developmental neurobiology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.716
H-Index - 129
eISSN - 1932-846X
pISSN - 1932-8451
DOI - 10.1002/dneu.22509
Subject(s) - zebrafish , biology , neurogenesis , wnt signaling pathway , neural stem cell , sox2 , neuroepithelial cell , microbiology and biotechnology , neuroscience , stem cell , neural development , notch signaling pathway , signal transduction , embryonic stem cell , genetics , gene
Adult neurogenesis occurs more commonly in teleosts, represented by zebrafish, than in mammals. Zebrafish is therefore considered a suitable model to study adult neurogenesis, for which the regulatory molecular mechanisms remain little known. Our previous study revealed that neuroepithelial‐like neural stem cells (NSCs) are located at the edge of the dorsomedial region. We also showed that Notch signaling inhibits NSC proliferation in this region. In the present study, we reported the expression of Wnt and Shh signaling components in this region of the optic tectum. Moreover, inhibitors of Wnt and Shh signaling suppressed NSC proliferation, suggesting that these pathways promote NSC proliferation. Shh is particularly required for maintaining Sox2‐positive NSCs. Our experimental data also indicate the involvement of these signaling pathways in neural differentiation from NSCs. © 2017 Wiley Periodicals, Inc. Develop Neurobiol 77: 1206–1220, 2017