z-logo
Premium
L mo4 and Other LIM domain only factors are necessary and sufficient for multiple retinal cell type development
Author(s) -
Jin Kangxin,
Xiao Dongchang,
Andersen Bogi,
Xiang Mengqing
Publication year - 2016
Publication title -
developmental neurobiology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.716
H-Index - 129
eISSN - 1932-846X
pISSN - 1932-8451
DOI - 10.1002/dneu.22365
Subject(s) - biology , microbiology and biotechnology , retina , retinal , lim domain , retinal ganglion cell , progenitor cell , cellular differentiation , amacrine cell , cell fate determination , cell type , cell , neuroscience , transcription factor , stem cell , genetics , gene , botany , zinc finger
Understanding the molecular basis by which distinct cell types are specified is a central issue in retinogenesis and retinal disease development. Here we examined the role of LIM domain only 4 (Lmo4) in retinal development using both gain‐of‐function and loss‐of‐function approaches. By immunostaining, Lmo4 was found to be expressed in mouse retina from E10.5 to mature stages. Retroviral delivery of Lmo4 into retinal progenitor cells could promote the amacrine, bipolar and Müller cell fates at the expense of photoreceptors. It also inhibited the fate of early‐born retinal ganglion cells. Using a dominant‐negative form of Lmo4 which suppresses transcriptional activities of all LIM domain only factors, we demonstrated that LIM domain only factors are both necessary and sufficient for promoting amacrine and bipolar cell development, but not for the differentiation of ganglion, horizontal, Müller, or photoreceptor cells. Taken together, our study uncovers multiple roles of Lmo4 during retinal development and demonstrates the importance of LIM domain only factors in ensuring proper retinal cell specification and differentiation. © 2015 Wiley Periodicals, Inc. Develop Neurobiol 76: 900–915, 2016

This content is not available in your region!

Continue researching here.

Having issues? You can contact us here