z-logo
Premium
S im1 is required for the migration and axonal projections of V3 interneurons in the developing mouse spinal cord
Author(s) -
Blacklaws Jake,
DeskaGauthier Dylan,
Jones Christopher T,
Petracca Yanina L.,
Liu Mingwei,
Zhang Han,
Fawcett James P.,
Glover Joel C.,
Lanuza Guillermo M.,
Zhang Ying
Publication year - 2015
Publication title -
developmental neurobiology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.716
H-Index - 129
eISSN - 1932-846X
pISSN - 1932-8451
DOI - 10.1002/dneu.22266
Subject(s) - commissure , biology , spinal cord , axon , neuroscience , anatomy , axon guidance , lumbar spinal cord , mutant , genetics , gene
ABSTRACT V3 spinal interneurons (INs) are a group of excitatory INs that play a crucial role in producing balanced and stable gaits in vertebrate animals. In the developing mouse spinal cord, V3 INs arise from the most ventral progenitor domain and form anatomically distinctive subpopulations in adult spinal cords. They are marked by the expression of transcription factor Sim1 postmitotically, but the function of Sim1 in V3 development remains unknown. Here, we used Sim1 Cre ;tdTomato mice to trace the fate of V3 INs in a Sim1 mutant versus control genetic background during development. In Sim1 mutants, V3 INs are produced normally and maintain a similar position and organization as in wild types before E12.5. Further temporal analysis revealed that the V3 INs in the mutants failed to migrate properly to form V3 subgroups along the dorsoventral axis of the spinal cord. At birth, in the Sim1 mutant the number of V3 INs in the ventral subgroup was normal, but they were significantly reduced in the dorsal subgroup with a concomitant increase in the intermediate subgroup. Retrograde labeling at lumbar level revealed that loss of Sim1 led to a reduction in extension of contralateral axon projections both at E14.5 and P0 without affecting ipsilateral axon projections. These results demonstrate that Sim1 is essential for proper migration and the guidance of commissural axons of the spinal V3 INs. © 2015 Wiley Periodicals, Inc. Develop Neurobiol 75: 1003–1017, 2015

This content is not available in your region!

Continue researching here.

Having issues? You can contact us here