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Dynamics of presynaptic protein recruitment induced by local presentation of artificial adhesive contacts
Author(s) -
Suarez Fernando,
Thostrup Peter,
Colman David,
Grutter Peter
Publication year - 2013
Publication title -
developmental neurobiology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.716
H-Index - 129
eISSN - 1932-846X
pISSN - 1932-8451
DOI - 10.1002/dneu.22037
Subject(s) - synaptophysin , neurite , axon , atomic force microscopy , biophysics , biology , neuroscience , synaptic vesicle , synapse , fluorescence microscope , nanotechnology , fluorescence , materials science , physics , vesicle , biochemistry , immunohistochemistry , membrane , immunology , in vitro , quantum mechanics
In this study, we introduce a novel approach to induce and observe the formation of presynaptic compartments in axons through a combination of atomic force microscopy (AFM) and fluorescence microscopy. First, we use a poly‐ D ‐lysine‐coated bead attached to an AFM tip to induce the recruitment of two synaptic proteins, bassoon and synaptophysin, and measure their absolute arrival times to the presynaptic department. We find that bassoon arrives before synaptophysin. Second, we observe the formation of very long (several 10s of μm), structured, protein‐containing membranous strings as the AFM tip was withdrawn from the axon. It is conceivable that these strings might be a novel mechanism by which new neurites or branch points along existing neurites may be generated in situ . © 2012 Wiley Periodicals, Inc. Develop Neurobiol, 2013