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Optogenetics: A new enlightenment age for zebrafish neurobiology
Author(s) -
Del Bene Filippo,
Wyart Claire
Publication year - 2012
Publication title -
developmental neurobiology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.716
H-Index - 129
eISSN - 1932-846X
pISSN - 1932-8451
DOI - 10.1002/dneu.20914
Subject(s) - optogenetics , zebrafish , neuroscience , biology , optical transparency , biological neural network , premovement neuronal activity , nervous system , gene , physics , biochemistry , optoelectronics
Zebrafish became a model of choice for neurobiology because of the transparency of its brain and because of its amenability to genetic manipulation. In particular, at early stages of development the intact larva is an ideal system to apply optical techniques for deep imaging in the nervous system, as well as genetically encoded tools for targeting subsets of neurons and monitoring and manipulating their activity. For these applications, new genetically encoded optical tools, fluorescent sensors, and light‐gated channels have been generated, creating the field of “optogenetics.” It is now possible to monitor and control neuronal activity with minimal perturbation and unprecedented spatio‐temporal resolution. We describe here the main achievements that have occurred in the last decade in imaging and manipulating neuronal activity in intact zebrafish larvae. We provide also examples of functional dissection of neuronal circuits achieved with the applications of these techniques in the visual and locomotor systems. © 2011 Wiley Periodicals, Inc. Develop Neurobiol 72: 404–414, 2012