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Effects of retinoic acid and butyric acid on the expression of prestin and gata‐3 in organotypic cultures of the organ of corti of newborn rats
Author(s) -
Gross Johann,
Stute Kerstin,
Fuchs Julia,
Angerstein Maximilian,
Amarjargal Nyamaa,
Mazurek Birgit
Publication year - 2011
Publication title -
developmental neurobiology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.716
H-Index - 129
eISSN - 1932-846X
pISSN - 1932-8451
DOI - 10.1002/dneu.20881
Subject(s) - prestin , retinoic acid , organ of corti , biology , microbiology and biotechnology , hes1 , histone deacetylase inhibitor , histone deacetylase , acetylation , histone , transcription factor , hair cell , biochemistry , cochlea , anatomy , notch signaling pathway , signal transduction , gene
Prestin is the motor protein of the outer hair cells of the organ of Corti and a key factor in ensuring a high level of sensitivity of mammalian hearing. The factors that influence prestin expression are still largely unknown. We studied the effects of the application of retinoic acid, a ligand of a nuclear receptor, and of butyric acid, an inhibitor of histone deacetylase activity, on the expression of mRNA of prestin and Gata‐3 in the organotypic culture of the organ of Corti of newborn rats using RT‐PCR. Application of retinoic acid at concentrations of 1–50 μ M results in a dose‐dependent expression decrease after two days in culture. Treatment with sodium butyrate (0.5–2 m M ) elevated the expression of prestin and Gata‐3. Statistically significant correlations between Gata‐3 and prestin mRNA levels were observed under all conditions. The data indicate that retinoid nuclear transcription factors, GATA‐3 and histone acetylation/deacetylation processes may have a regulatory role to play in prestin expression. © 2010 Wiley Periodicals, Inc. Develop Neurobiol 71: 650–661, 2011