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Gap junctions are involved in cell migration in the early postnatal subventricular zone
Author(s) -
Marins Mônica,
Xavier Anna L.R.,
Viathan B.,
Fortes Fábio S.A.,
Fróes Maira M.,
Menezes João R.L.
Publication year - 2009
Publication title -
developmental neurobiology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.716
H-Index - 129
eISSN - 1932-846X
pISSN - 1932-8451
DOI - 10.1002/dneu.20737
Subject(s) - subventricular zone , neuroblast , rostral migratory stream , gap junction , biology , neuroscience , carbenoxolone , cell migration , microbiology and biotechnology , neurogenesis , cell , anatomy , stem cell , neural stem cell , intracellular , genetics
The massive migration of neuroblasts and young neurons through the anterior extension of the postnatal subventricular zone (SVZ), known as the rostral migratory stream (RMS) is still poorly understood on its molecular basis. In this work, we investigated the involvement of gap junctional communication (GJC) in the robust centrifugal migration from SVZ/RMS explants obtained from early postnatal (P4) rats. Cells were dye‐coupled in homocellular and heterocellular pairings and expressed at least two connexins, Cx 43 and 45. Treatment with the uncoupler agent carbenoxolone (CBX, 10–100 μ M ) reversibly reduced outgrowth from SVZ explants, while its inactive analog, glycyrhizinic acid (GZA), had no effect. Consistent with a direct effect on cell migration, time‐lapse video microscopy show that different pharmacological uncouplers cause an abrupt and reversible arrest of cell movement in explants. Our results indicate that GJC is positively involved in the migration of neuroblasts within the SVZ/RMS. © 2009 Wiley Periodicals, Inc. Develop Neurobiol, 2009