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Normal dendrite growth in Drosophila motor neurons requires the AP‐1 transcription factor
Author(s) -
Hartwig Cortnie L.,
Worrell Jason,
Levine Richard B.,
Ramaswami Mani,
Sanyal Subhabrata
Publication year - 2008
Publication title -
developmental neurobiology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.716
H-Index - 129
eISSN - 1932-846X
pISSN - 1932-8451
DOI - 10.1002/dneu.20655
Subject(s) - biology , dendrite (mathematics) , neuroscience , transcription factor , soma , biological neural network , microbiology and biotechnology , synapse , premovement neuronal activity , neuron , neural development , depolarization , gene , genetics , biophysics , geometry , mathematics
During learning and memory formation, information flow through networks is regulated significantly through structural alterations in neurons. Dendrites, sites of signal integration, are key targets of activity‐mediated modifications. Although local mechanisms of dendritic growth ensure synapse‐specific changes, global mechanisms linking neural activity to nuclear gene expression may have profound influences on neural function. Fos, being an immediate‐early gene, is ideally suited to be an initial transducer of neural activity, but a precise role for the AP‐1 transcription factor in dendrite growth remains to be elucidated. Here we measure changes in the dendritic fields of identified Drosophila motor neurons in vivo and in primary culture to investigate the role of the immediate‐early transcription factor AP‐1 in regulating endogenous and activity‐induced dendrite growth. Our data indicate that (a) increased neural excitability or depolarization stimulates dendrite growth, (b) AP‐1 (a Fos, Jun hetero‐dimer) is required for normal motor neuron dendritic growth during development and in response to activity induction, and (c) neuronal Fos protein levels are rapidly but transiently induced in motor neurons following neural activity. Taken together, these results show that AP‐1 mediated transcription is important for dendrite growth, and that neural activity influences global dendritic growth through a gene‐expression dependent mechanism gated by AP‐1. © 2008 Wiley Periodicals, Inc. Develop Neurobiol, 2008