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A unique cell population in the mouse olfactory bulb displays nuclear β‐catenin signaling during development and olfactory sensory neuron regeneration
Author(s) -
BookerDwyer Tiara,
Hirsh Sarah,
Zhao Haiqing
Publication year - 2008
Publication title -
developmental neurobiology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.716
H-Index - 129
eISSN - 1932-846X
pISSN - 1932-8451
DOI - 10.1002/dneu.20606
Subject(s) - olfactory bulb , biology , olfactory ensheathing glia , wnt signaling pathway , olfactory epithelium , neuroscience , olfactory system , microbiology and biotechnology , sensory system , population , regeneration (biology) , central nervous system , signal transduction , demography , sociology
Abstract Olfactory sensory neurons (OSNs) in the nose form precise connections with neurons in the brain. However, mechanisms that account for the formation of such precise neuronal connections are incompletely understood. Recent studies implicate the function of Wnt growth factors in the formation of neuronal connections. To assess the role of Wnt signaling in the olfactory system, we examined the expression of β‐galactosidase (β‐gal) in the TOPGAL mouse, a transgenic strain in which β‐gal expression reports the activation of the canonical Wnt signaling pathway. In the olfactory epithelium, no β‐gal expression was observed at any developmental stages. In the olfactory bulb (OB), β‐gal expression was observed in a population of cells located at the interface of the olfactory nerve layer and the glomerular layer. The β‐gal expression was developmentally regulated with the peak expression occurring at late embryonic and early postnatal stages and a greatly reduced expression in adulthood. Further, forced OSN regeneration and subsequent reinnervation of the OB led to a reactivation of β‐gal expression in mature animals. The temporal coincidence between the peak of β‐gal expression and formation of OSN connections, together with the spatial localization of these cells, suggests a potential role of these cells and canonical Wnt signaling in the formation of OSN connections in the OB during development and regeneration. © 2008 Wiley Periodicals, Inc. Develop Neurobiol, 2008.