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Costorage of BDNF and neuropeptides within individual dense‐core vesicles in central and peripheral neurons
Author(s) -
Salio C.,
Averill S.,
Priestley J.V.,
Merighi A.
Publication year - 2007
Publication title -
developmental neurobiology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.716
H-Index - 129
eISSN - 1932-846X
pISSN - 1932-8451
DOI - 10.1002/dneu.20358
Subject(s) - neuropeptide , neuroscience , brain derived neurotrophic factor , neurotrophic factors , biology , calcitonin gene related peptide , substance p , spinal cord , immunocytochemistry , endocrinology , medicine , microbiology and biotechnology , receptor , biochemistry
Some central and peripheral neurons synthesize brain‐derived neurotrophic factor (BDNF), and, after anterograde transport, release it at synapses. By immunocytochemistry, we examined, in rat and mouse, the subcellular localization of BDNF and BDNF/peptide coexistence, under normal conditions or after intrathecal infusion of nerve growth factor. In dorsal root ganglion neurons and afferent terminals, and in the parabrachial projection to amygdala, we show that BDNF is costored in individual dense‐core vesicles (DCVs) with the neuropeptides calcitonin gene‐related peptide (CGRP) and substance P. At both locations, nerve endings costoring all three peptides were fairly rare. Remarkably however, costorage occurred in a stoichiometric ratio of 0.7 BDNF:1 CGRP:1 substance P, and DCVs contained 31 (spinal cord) −36 (amygdala) times the amount of BDNF detected in agranular vesicles. This is the first direct demonstration in peripheral and central neurons from two different mammals, that a growth factor is selectively packaged together with neuropeptide transmitters within individual DCVs. It provides structural bases for differential release upon stimulation, and has important implications for understanding BDNF transmitter function. © 2007 Wiley Periodicals, Inc. Develop Neurobiol, 2007