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Changes in cell migration and survival in the olfactory bulb of the pcd/pcd mouse
Author(s) -
Valero J.,
Weruaga E.,
Murias A.R.,
Recio J.S.,
Curto G.G.,
Gómez C.,
Alonso J.R.
Publication year - 2007
Publication title -
developmental neurobiology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.716
H-Index - 129
eISSN - 1932-846X
pISSN - 1932-8451
DOI - 10.1002/dneu.20352
Subject(s) - neuroblast , olfactory bulb , biology , rostral migratory stream , bromodeoxyuridine , neuroscience , progenitor cell , microbiology and biotechnology , neurogenesis , central nervous system , stem cell , subventricular zone , immunology , immunohistochemistry
Abstract Postnatally, the Purkinje cell degeneration mutant mice lose the main projecting neurons of the main olfactory bulb (OB): mitral cells (MC). In adult animals, progenitor cells from the rostral migratory stream (RMS) differentiate into bulbar interneurons that modulate MC activity. In the present work, we studied changes in proliferation, tangential migration, radial migration patterns, and the survival of these newly generated neurons in this neurodegeneration animal model. The animals were injected with bromodeoxyuridine 2 weeks or 2 months before killing in order to label neuroblast incorporation into the OB and to analyze the survival of these cells after differentiation, respectively. Both the organization and cellular composition of the RMS and the differentiation of the newly generated neurons in the OB were studied using specific markers of glial cells, neuroblasts, and mature neurons. No changes were observed in the cell proliferation rate nor in their tangential migration through the RMS, indicating that migrating neuroblasts are only weakly responsive to the alteration in their target region, the OB. However, the absence of MC does elicit differences in the final destination of the newly generated interneurons. Moreover, the loss of MC also produces changes in the survival of the newly generated interneurons, in accordance with the dramatic decrease in the number of synaptic targets available. © 2007 Wiley Periodicals, Inc. Develop Neurobiol, 2007.