Premium
A novel model of insulin‐dependent diabetes with renal and retinal lesions by transgenic expression of CaMKIIα (Thr286Asp) in pancreatic β‐cells
Author(s) -
Kato Ichiro,
Oya Takeshi,
Suzuki Hikari,
Takasawa Kumi,
Ichsan Andi M.,
Nakada Shinji,
Ishii Yoko,
Shimada Yutaka,
Sasahara Masakiyo,
Tobe Kazuyuki,
Takasawa Shin,
Okamoto Hiroshi,
Hiraga Koichi
Publication year - 2008
Publication title -
diabetes/metabolism research and reviews
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.307
H-Index - 110
eISSN - 1520-7560
pISSN - 1520-7552
DOI - 10.1002/dmrr.864
Subject(s) - medicine , endocrinology , insulin , pancreatic islets , diabetes mellitus , genetically modified mouse , streptozotocin , biology , in vivo , beta cell , pancreas , chemistry , transgene , islet , biochemistry , microbiology and biotechnology , gene
Background The activation of Ca 2+ /calmodulin‐dependent protein kinase II (CaMKII) in pancreatic β‐cells has been thought to play a central role in Ca 2+ ‐mediated insulin secretion. However, the physiological and pathological significance of CaMKII activation in pancreatic β‐cells has never been investigated in vivo . Methods We generated transgenic (TG) mice overexpressing the constitutively active‐type CaMKIIα (Thr286Asp) in β‐cells. The mice were extensively examined histologically and biochemically. Time‐course changes of blood glucose, haemoglobin A 1C and insulin were also determined. Results Western blot and immunohistochemical analyses showed overexpression of CaMKIIα proteins in pancreatic β‐cells of TG mice. All TG mice developed severe hypoinsulinaemic diabetes by P28. In vivo BrdU labelling analysis revealed that cell proliferation in TG islets is severely impaired. Immunohistochemical examination revealed accumulations of NF‐κB in nuclei of TG β‐cells at P21, which are associated with DNA laddering, a hallmark of apoptosis. At P28, pancreatic and serum insulin levels were both significantly ( p < 0.05) lower in TG mice (0.037 ± 0.005 ng/µ g and 0.50 ± 0.01 ng/mL) than in wild‐type mice (0.997 ± 0.093 ng/µ g and 2.50 ± 0.22 ng/mL). TG mice at P140 showed enlargement of kidney, mesangial expansion and glomerulosclerosis, which are associated with urinary albumin excretion. TG mice at P140‐P168 developed severe retinal lesions such as disrupted ganglion cells and showed a flat pattern in electroretinography. Conclusions The TG mice established herein will be valuable as a novel model of severe insulin‐dependent diabetes accompanied by an early progression of diabetic micro‐vascular complications. Copyright © 2008 John Wiley & Sons, Ltd.